Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell function-based flow cytometry.

J J Bleesing1, T A Fleisher

  • 1Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508, USA.

Seminars in Hematology
|April 20, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 20 Year Clinical and Laboratory Study of Familial B-Chronic Lymphocytic Leukemia in a Single Kindred.

Leukemia & lymphoma·2016
Same author

Loss of B Cells in Patients with Heterozygous Mutations in IKAROS.

The New England journal of medicine·2016
Same author

Activated STING in a vascular and pulmonary syndrome.

The New England journal of medicine·2014
Same author

Alternative donor SCT for the treatment of MHC class II deficiency.

Bone marrow transplantation·2012
Same author

Outcomes following hematopoietic cell transplantation for Wiskott-Aldrich syndrome.

Bone marrow transplantation·2012
Same author

The relationship between alloimmunization and posttransfusion granulocyte survival: experience in a chronic granulomatous disease cohort.

Transfusion·2010

Flow cytometry now assesses leukocyte function, revealing insights into cell processes and disease states. Advanced techniques like MHC-peptide tetramers enable detailed study of T cells, enhancing immunology understanding.

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry is a key tool for analyzing leukocyte function.
  • It examines cell surface markers, intracellular processes, and cell viability.
  • Multiparameter analysis offers comprehensive cellular insights.

Purpose of the Study:

  • To highlight the expanding role of flow cytometry in immunology.
  • To discuss advancements in functional cell analysis.
  • To explore the impact of new technologies on understanding immune responses.

Main Methods:

  • Utilizing multiparameter flow cytometry for immunophenotyping.
  • Employing functional assays to assess cellular processes like cytokine production and phosphorylation.
  • Applying major histocompatibility complex (MHC)-peptide tetramer technology for antigen-specific T cell detection.

Related Experiment Videos

Main Results:

  • Flow cytometry provides deep insights into lymphocyte function and disease.
  • MHC-peptide tetramer technology allows direct visualization and quantification of specific T cells.
  • These methods enhance understanding of basic and clinical immunology.

Conclusions:

  • Flow cytometry is indispensable for studying leukocyte function and immune cell dynamics.
  • Advanced techniques are revolutionizing the study of T cell subsets and immune responses.
  • This technology prompts a re-evaluation of established immunological concepts.