Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Semi-automatized processing of AFM force-spectroscopy data.

C Gergely1, B Senger, J C Voegel

  • 1Institut National de la Santé et de la Recherche Médicale, Unité 424, Fédération de Recherches Odontoloqie, Université Louis Pasteur, Strasbourg, France.

Ultramicroscopy
|April 20, 2001
PubMed
Summary

This study introduces a computer algorithm to analyze complex atomic force microscopy (AFM) force curves, improving the reproducible measurement of single-molecule rupture forces in ligand-receptor interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute Traumatic Subdural Hematoma in the Elderly and Associated Factors that May Influence Chronicity.

The American surgeon·2023
Same author

Modulation of Cellular Colonization of Porous Polyurethane Scaffolds via the Control of Pore Interconnection Size and Nanoscale Surface Modifications.

ACS applied materials & interfaces·2019
Same author

Step-by-step build-up of covalent poly(ethylene oxide) nanogel films.

Nanoscale·2017
Same author

Bioinspired Nanofeatured Substrates: Suitable Environment for Bone Regeneration.

ACS applied materials & interfaces·2017
Same author

Harnessing Wharton's jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors.

Acta biomaterialia·2016
Same author

Laboratory Studies of Nonlinear Optical Signals for Caries Detection.

Journal of dental research·2016

Area of Science:

  • Biophysics
  • Surface Science
  • Materials Science

Background:

  • Atomic force microscopy (AFM) in force-spectroscopy mode is crucial for measuring single-molecule interactions.
  • Complex force curves arise from simultaneous interactions, complicating analysis, especially for non-specific adhesion.
  • Manual analysis of force curves can be subjective and time-consuming.

Purpose of the Study:

  • To develop a computer algorithm for automated and reproducible analysis of AFM force curves.
  • To address challenges in identifying specific molecular events amidst complex adhesion signals.
  • To standardize the evaluation of rupture forces from single-molecule force spectroscopy experiments.

Main Methods:

  • Development and application of a novel computer algorithm for processing AFM force-spectroscopy data.

Related Experiment Videos

  • Analysis of force recordings obtained at various retraction velocities (loading rates).
  • Investigation of the impact of operator-set parameters on rupture force evaluation.

    • Main Results:

    • The proposed algorithm automates the processing of complex force curves, enhancing reproducibility.
    • It enables more reliable identification of rupture events, even with non-specific molecular adhesion.
    • The study discusses the influence of experimental parameters on the accuracy of rupture force measurements.

    Conclusions:

    • The developed algorithm significantly improves the efficiency and objectivity of AFM force-spectroscopy data analysis.
    • This tool facilitates more accurate and reproducible quantification of single-molecule interactions.
    • It provides a standardized approach for evaluating rupture forces across different experimental conditions.