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Updated: Jul 28, 2026

Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
The kinetics of hypoxanthine efflux from the rat brain
Z B Redzic1, A Isakovic, M B Segal
1Institute of Biochemistry, School of Medicine, Pasterova 2, Belgrade, Yugoslavia.
Abstract:
The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [K(eff)(i)=0.21+/-0.06 min(-1)], which was saturable with a V(max)=13.08+/-0.81 nM min(-1) g(-1), and a high K(m,app) (67.2+/-13.4 microM); the K(i,app) for inosine was 31.5+/-7.6 microM. Capillary depletion analysis indicated that hypoxanthine accumulates in neurons and glia with the time. From cross-inhibition studies with different purines and pyrimidines, it suggests that these molecules could also be important substrates for this carrier.
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