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A fibre-optic calcium ion sensor using a calcein derivative.

W D Sloan1, M Uttamlal

  • 1Department of Physical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.

Luminescence : the Journal of Biological and Chemical Luminescence
|April 20, 2001
PubMed
Summary

A novel fiber-optic calcium ion sensor was developed using immobilized calcein acrylamide (CA). This sensor offers sensitive detection of calcium ions, crucial for various analytical applications.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Accurate calcium ion (Ca2+) detection is vital in biological and chemical analyses.
  • Existing Ca2+ sensors may have limitations in sensitivity, selectivity, or operational pH range.
  • Development of robust and sensitive optical sensors for Ca2+ remains an active research area.

Purpose of the Study:

  • To develop a new fiber-optic sensor for sensitive detection of calcium ions (Ca2+).
  • To immobilize a calcium-sensitive dye, calcein acrylamide (CA), onto an optical fiber for enhanced stability and usability.
  • To characterize the performance of the developed sensor, including its sensitivity, linear range, and operational pH.

Main Methods:

  • Covalent immobilization of calcein acrylamide (CA) onto an optical fiber using a polymer matrix.

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  • Preparation of the calcium-sensitive polymer via photo-initiated polymerization of acrylamide and acrylamide doped with MAPTAC.
  • Characterization of the sensor's fluorescence response to varying Ca2+ concentrations and pH levels.
  • Investigation of Ca2+ binding stoichiometry at different concentration ranges.

    • Main Results:

    • The developed fiber-optic sensor demonstrated pH sensitivity between pH 4-12.
    • Fluorescence intensity increased with Ca2+ concentration at pH > 12, with a sensing range of 0-20 µmol/L.
    • A linear response was observed for Ca2+ concentrations up to the CA concentration, with a 1:1 binding ratio.
    • Polymers doped with MAPTAC enabled Ca2+ analysis at pH 8.5, extending the sensing range to 0-12 µmol/L.
    • Fluorescence characteristics of immobilized CA were comparable to CA in solution.

    Conclusions:

    • The novel fiber-optic sensor provides a sensitive and robust platform for Ca2+ detection.
    • The sensor's performance can be tuned by polymer composition, allowing for analysis in different pH environments.
    • This technology holds potential for applications in environmental monitoring, biological research, and clinical diagnostics requiring precise Ca2+ measurements.