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Genetic markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) obtained by the double stringency
1Laboratório de Malacologia, Superintendência de Controle de Endemias, São Paulo, SP, 01027-000, Brasil. tuan@sucen.sp.gov.br
Abstract:
Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.
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