Characterization and quantitation of the five major plasma protein fractions in seven-day-old piglets

Cellulose acetate electrophoresis was applied to resolve, characterize and quantitate the five major plasma protein fractions of 7-day-old pigs. Purified Cohn fractions were added to the piglets plasma and the individual fractions characterized by similarities in mobilities. All mobilities were expressed relative to albumin. Relative percentages and concentrations of the electrophoretically separated plasma proteins were also calculated. The respective mean values for the plasma concentration of albumin, alpha-, beta, gamma-globulin and fibrinogen obtained from 45 piglets were 1.98, 0.93, 0.27, 0.60 and 1.49 g/100 ml, respectively.