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Targeted gene correction by small single-stranded oligonucleotides in mammalian cells

O Igoucheva1, V Alexeev, K Yoon

  • 1Department of Dermatology and Cutaneous Biology, Department of Biochemistry and Molecular Pharmacology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University and Jefferson Medical College, Philadelphia, PA 19107, USA.

Gene Therapy
|April 21, 2001
PubMed
Summary

Short single-stranded oligodeoxynucleotides (ODN) can correct single point mutations in mammalian cells. These ODNs demonstrate gene correction frequencies comparable to chimeric RNA-DNA oligonucleotides.

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