Related Experiment Video

Updated: Aug 7, 2026

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

The chicken Pdcd4 gene is regulated by v-Myb

U Schlichter1, O Burk, S Worpenberg

  • 1Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany.

Oncogene
|April 21, 2001
PubMed

Insights

The avian myeloblastosis virus oncogene v-myb transforms cells by disrupting differentiation. This study identifies the chicken Pdcd4 gene as a novel v-Myb target, revealing v-Myb regulates a potential tumor suppressor.

Area of Science:

  • Molecular Biology
  • Oncology
  • Virology

Background:

  • The retroviral oncogene v-myb drives avian myeloblastosis virus (AMV) transformation of myelomonocytic cells.
  • v-Myb is hypothesized to impede cell differentiation by altering target gene expression.

Purpose of the Study:

  • To identify genes regulated by v-Myb in myelomonocytic cells.
  • To investigate the role of v-Myb in disrupting cellular differentiation.

Main Methods:

  • Differential display analysis of gene expression in a chicken myelomonocytic cell line.
  • Utilized an estrogen-inducible v-Myb system for gene expression modulation.

Main Results:

  • Identified the chicken homolog of the mouse Pdcd4 gene (also known as MA-3, TIS, H731) as a novel v-Myb target gene.
  • Pdcd4 has a known role in suppressing epidermal cell transformation by tumor promoters.
  • Demonstrated direct regulation of Pdcd4 expression by v-Myb.

Conclusions:

  • v-Myb directly regulates the expression of the Pdcd4 gene.
  • This finding suggests v-Myb may disrupt myelomonocytic cell differentiation by downregulating a potential tumor suppressor gene.

More Related Videos

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...