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Updated: Aug 7, 2026
11:19
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
Published on: July 7, 2010
The chicken Pdcd4 gene is regulated by v-Myb
U Schlichter1, O Burk, S Worpenberg
1Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany.
Oncogene
|April 21, 2001
Summary
The avian myeloblastosis virus oncogene v-myb transforms cells by disrupting differentiation. This study identifies the chicken Pdcd4 gene as a novel v-Myb target, revealing v-Myb regulates a potential tumor suppressor.
Area of Science:
- Molecular Biology
- Oncology
- Virology
Background:
- The retroviral oncogene v-myb drives avian myeloblastosis virus (AMV) transformation of myelomonocytic cells.
- v-Myb is hypothesized to impede cell differentiation by altering target gene expression.
Purpose of the Study:
- To identify genes regulated by v-Myb in myelomonocytic cells.
- To investigate the role of v-Myb in disrupting cellular differentiation.
Main Methods:
- Differential display analysis of gene expression in a chicken myelomonocytic cell line.
- Utilized an estrogen-inducible v-Myb system for gene expression modulation.
Main Results:
- Identified the chicken homolog of the mouse Pdcd4 gene (also known as MA-3, TIS, H731) as a novel v-Myb target gene.
- Pdcd4 has a known role in suppressing epidermal cell transformation by tumor promoters.
- Demonstrated direct regulation of Pdcd4 expression by v-Myb.
Conclusions:
- v-Myb directly regulates the expression of the Pdcd4 gene.
- This finding suggests v-Myb may disrupt myelomonocytic cell differentiation by downregulating a potential tumor suppressor gene.
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