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In vitro cytotoxicity evaluation of denture adhesive
1Department of Prosthodontics, School of Stomatology, Wuhan University, No. 237 Luoyu Road, Wuhan 430079, P.R. China.
Objective:
The ideal denture adhesive demonstrates a minimal toxicity to the mucosa. The purpose of this study was to evaluate the in vitro cytotoxicity of a new denture adhesive.
Material And Methods:
Samples were prepared and extracted in Dulbecco's Modified Eagle's Medium. The cell viability of human oral fibroblast cells cultivated for 24 hours with DA-containing medium was evaluated by the MTT assay to investigate cell proliferation. Optical densities were measured by a spectrophotometer at 490 nm.
Results:
The statistical analysis of results showed that this denture adhesive displayed only slight cytotoxicity.
Conclusion:
The generally favorable in vitro cytotoxicity of the denture adhesive formulation indicates that this product may be efficacious.