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Related Experiment Videos

Decreasing the variability observed in urine analysis.

Z K Shihabi1, R P Schwartz, M J Pugia

  • 1Department of Pathology, Wake Forest University Baptist Medical Center, Winston-Salem, North Carolina, USA. zshihabi@wfubmc.edu

Annals of Clinical and Laboratory Science
|April 21, 2001
PubMed
Summary

Mixing early morning urine samples reduces biological variability in children with diabetes. This method improves the accuracy of urine albumin and glucose measurements, aiding in better diabetes management.

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Area of Science:

  • Clinical Chemistry
  • Pediatric Nephrology
  • Diabetology

Background:

  • Biological variability significantly impacts urine analysis accuracy.
  • Accurate monitoring of urine analytes is crucial for managing pediatric diabetes.
  • High variability in urine albumin excretion is a concern in diabetic children.

Purpose of the Study:

  • To assess the feasibility of reducing urine analyte biological variability by mixing early morning samples.
  • To investigate the impact of sample mixing on urine albumin and glucose measurements in children with type 1 diabetes.
  • To evaluate the correlation between blood glycated hemoglobin and mixed urine glucose levels.

Main Methods:

  • Collected early morning urine aliquots (approx. 10 ml) from 22 male children with type 1 diabetes over 3 consecutive days.

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  • Included 10 normal children and 10 normal adults as controls.
  • Analyzed individual and mixed (pooled 3-day samples) urine specimens for albumin, glucose, creatinine, total protein, and potassium.

    • Main Results:

    • Mixing urine samples significantly decreased biological variability for all tested analytes.
    • Diabetic children showed 3 times higher urine albumin-to-creatinine ratio variability (61%) compared to controls (19%) with individual samples.
    • Variability in diabetic children decreased upon mixing samples, particularly for albumin-to-creatinine ratio.
    • Mixing samples improved the correlation between blood glycated hemoglobin and urine glucose levels.

    Conclusions:

    • Mixing early morning urine samples is a feasible method to reduce biological variability in pediatric urine analysis.
    • This technique enhances the reliability of urine albumin and glucose measurements in children with diabetes.
    • Sample mixing offers a potential strategy for more accurate diabetes monitoring in children.