Related Experiment Videos
Inhibitors of platelet lipoxygenase from Ponkan fruit
1Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Fukuyama, Hiroshima, Japan. ynogata@cgk.affrc.go.jp
Phytochemistry
|April 21, 2001
Abstract:
An activity-guided separation for inhibitors of rat platelet 12-lipoxygenase led to the isolation of two compounds, 4-O-feruloyl-5-O-caffeoylquinic acid (IC50; 5.5 microM) and methyl 4-O-feruloyl-5-O-caffeoylquinate (IC50; 1.9 microM) from the peel of Ponkan fruit (Citrus reticulata). The complete structure of each phenolic ester was determined by NMR spectroscopy [1H and 13C NMR spectra, 1H-1H correlation spectroscopy (COSY), 1H-detected heteronuclear multiple quantum coherence (HMQC), and heteronuclear multiple bond connectivity (HMBC) spectroscopies] and other spectral methods.