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Survival and transmissibility of Pasteurella pneumotropica
1Bundesinstitut für Gesundheitlichen Verbraucherschutz und Veterinärmedizin, Fachgebiet Versuchstierkunde, Berlin.
Abstract:
Survival has been determined for Pasteurella pneumotropica on various surfaces found in an animal room at 23+/-1 degrees C and 50+/-10% relative humidity. Longest survival (120 min) was found on mouse hair, shortest (< 30 min) on laboratory coat fabric. Transmission experiments were performed using sentinel animals in order to evaluate the efficiency of their use for the detection of P. pneumotropica in quarantined mice. In sentinels exposed to infected mice by close contact, P. pneumotropica was detected by culture 2 weeks post-exposure and seroconversion 3 weeks after contact. Transfer of soiled bedding from Pasteurella-infected mice did not infect sentinels within a period of 12 weeks as tested by cultivation or serum antibodies.
Insights
Pasteurella pneumotropica survives longest on mouse hair (120 min) but briefly on lab coats (<30 min). Sentinel mice detected transmission via direct contact but not soiled bedding, indicating limited environmental spread.
Area of Science:
- Microbiology
- Animal Health
- Infectious Disease Transmission
Background:
- Pasteurella pneumotropica is a common bacterium in laboratory animals.
- Understanding its survival on surfaces is crucial for infection control in animal facilities.
- Effective detection methods are needed to manage potential outbreaks.
Purpose of the Study:
- To determine the survival time of Pasteurella pneumotropica on various animal room surfaces.
- To evaluate the efficacy of sentinel animals for detecting P. pneumotropica in quarantined mice.
- To assess transmission routes, including direct contact and soiled bedding.
Main Methods:
- Survival assays of P. pneumotropica on different surfaces at controlled temperature and humidity.
- Transmission experiments using sentinel mice exposed to infected mice via direct contact.
- Transmission experiments using sentinel mice exposed to soiled bedding from infected mice.
- Detection of P. pneumotropica using culture and seroconversion in sentinel animals.
Main Results:
- Pasteurella pneumotropica exhibited variable survival times, with the longest on mouse hair (120 minutes) and shortest on laboratory coat fabric (<30 minutes).
- Sentinel mice exposed to infected mice by direct contact were positive for P. pneumotropica via culture at 2 weeks and seroconversion at 3 weeks.
- Exposure to soiled bedding from infected mice did not result in P. pneumotropica detection in sentinel animals within 12 weeks.
Conclusions:
- P. pneumotropica has limited survival on common animal room surfaces, particularly fabric.
- Sentinel animals are effective in detecting P. pneumotropica transmission through direct contact with infected animals.
- Transmission via soiled bedding appears to be an inefficient route for P. pneumotropica spread in this model.