Related Experiment Video
Updated: Oct 8, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Effect of culture conditions on lipase production by Fusarium solani in batch fermentation
M M Maia1, A Heasley, M M Camargo de Morais
1Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, Brazil.
Abstract:
Lipase (Glycerol ester hydrolase EC 3.1.1.3.) from a Brazilian strain of Fusarium solani FSI has been investigated. The effect of different carbon sources and trace elements added to basal medium was observed with the aim of improving enzyme production. Lipase specific activity was highest (0.45 U mg(-1)) for sesame oil. When this medium was supplemented with trace elements using olive oil, corn oil and sesame oil the lipase specific activity increased to 0.86, 1.89 and 1.64 U mg(-1), respectively, after 96 h cultivation without any considerable biomass increase. The Km of this lipase using pNPP (p-nitrophenylpalmitate) as substrate, was 1.8 mM with a Vmax of 1.7 micromol min(-1) mg protein(-1). Lipase activity increased in the presence of increasing concentrations of hexane and toluene. In contrast, incubation of this enzyme with water-soluble solvents decreased its activity after 10% concentration (v/v) of the solvent. The lipase activity was stable below 35 degrees C but above this temperature activity losses were observed.
Related Concept Videos
Batch vs Continuous Culture
Fed-Batch Culture
Microbes in Food Production
Scale-Up Processes
Microbes in the Production of Fermented Foods
Production of Organic Acids