Morphological and cytochemical study of extracellular matrix during the migratory phase of human and mouse primordial...

Primordial germ cells (PGCs), the ancestors of functional gametes in mammals, originate in an extragonadal location, and then migrate to and colonize the genital ridges during early organogenesis period. PGCs move actively from their original site, the wall of the hindgut, through the extracellular matrix (ECM) of the dorsal mesentery. This movement is controlled in part by components of the ECM. Cells are known to bind to individual ECM glycoproteins in a complex and poorly understood way. During migration in embryos, PGCs must alter their overall adhesiveness to the endodermal epithelium to allow locomotion. This study examined the ECM material of the migratory route during mouse and human PGCs migration. Mouse embryos obtained from Swiss Rockefeller mouse and normal human embryos between 4 and 7 weeks of development, collected during salpingectomy performed on patients with tubal ectopic pregnancies, were analyzed. The study was based on a morphological analysis using scanning electron microscopy (SEM), and on the histochemical and ultracytochemical identification of glycosaminoglycans (GAGs) and proteoglycans. In each age group, the mesenchyme was widely separated by intercellular spaces and materials. Fine filamentous strands extended between the surface of mesenchymal cells and the surface of PGCs. Hyaluronan and chondroitin and/or dermatan sulfate were localized in the ECM of the PGC migratory pathway both in mouse and human embryos. Hyaluronan was clearly reduced in the later stage of the migratory processes; on the contrary, the chondroitin sulfate reaction product increased. These results are consistent with previous observations showing that hyaluronan is a major component of the ECM, and are also suggestive of the significant role played by hyaluronan, chondroitin sulfate and dermatan sulfate during migration, thus providing a permissive substrate for cell migration during development. The observed temporal and regional patterns suggest that these GAGs are important morphogenetic factors both in the mouse and human although the precise biological function of the proteoglycans are not currently clear.