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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

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Molecular characterization of an endopolygalacturonase from Fusarium oxysporum expressed during early stages of

F I García-Maceira¹, A Di Pietro, M D Huertas-González

¹Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba, Avda. San Alberto Magno s/n, 14071 Córdoba, Spain.

Applied and Environmental Microbiology

|April 25, 2001

Summary

Fusarium oxysporum f. sp. lycopersici produces a novel pectinolytic enzyme, pg5, which is expressed during tomato root infection. However, inactivating pg5 did not affect the pathogen

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Published on: September 30, 2016

Area of Science:

Plant Pathology
Mycology
Molecular Biology

Background:

Fusarium oxysporum f. sp. lycopersici causes vascular wilt in tomato plants.
Pectinolytic enzymes secreted by F. oxysporum may aid in host plant penetration and colonization.

Purpose of the Study:

To isolate and characterize a novel pectinolytic enzyme gene, pg5, from F. oxysporum.
To investigate the role of pg5 in the pathogenesis of tomato vascular wilt.

Main Methods:

Isolation and cloning of the pg5 gene.
Heterologous expression of pg5 in a bacterial system.
Reverse transcription-PCR to analyze pg5 expression during infection.

Main Results:

A novel extracellular endopolygalacturonase (endoPG) gene, pg5, was identified and found to be conserved across F. oxysporum formae speciales.
The pg5 gene product exhibits endoPG activity and its transcript accumulation is regulated by pectin and D-galacturonic acid.
pg5 is expressed in tomato roots during early infection stages, but its inactivation did not impact virulence.

Conclusions:

pg5 encodes a conserved endoPG in F. oxysporum with pectin-regulated expression.
Despite early expression during infection, pg5 is not essential for the virulence of F. oxysporum on tomato plants.