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Related Experiment Videos

Receptor-targeted gene delivery using multivalent phagemid particles.

D Larocca1, K Jensen-Pergakes, M A Burg

  • 1Selective Genetics, Inc., 11035 Roselle Street, San Diego, California 92121, USA. laroccad@selectivegenetics.com

Molecular Therapy : the Journal of the American Society of Gene Therapy
|April 26, 2001
PubMed
Summary
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Multivalent phagemid vectors enhance mammalian cell transduction efficiency by displaying epidermal growth factor (EGF). This improved phage vector system shows promise for gene therapy and ligand discovery applications.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Therapy

Background:

  • Filamentous phage vectors can transduce mammalian cells but require efficiency improvements for gene therapy.
  • Targeted phage vectors are valuable for gene therapy and ligand discovery.

Purpose of the Study:

  • To develop and evaluate multivalent phagemid vectors for enhanced ligand-targeted mammalian cell transduction.
  • To optimize the display of epidermal growth factor (EGF) on phagemid vectors.

Main Methods:

  • Constructed multivalent phagemid vectors with EGF fused to the pIII coat protein.
  • Compared phagemid particles with high and low EGF valence using helper phage.
  • Utilized immunoblotting to assess protein display and measured transduction efficiency.

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Main Results:

  • Phagemid vectors demonstrated advantages over phage vectors in insert size and stability.
  • Optimized EGF fusion to the pIII coat protein resulted in highly efficient multivalent display.
  • Increased EGF display correlated with enhanced cell internalization, vector potency, and approximately 9% transduction efficiency.

Conclusions:

  • Multivalent phagemid vectors significantly improve mammalian cell transduction efficiency.
  • Phage-based vector systems can be modified for more effective gene transfer.
  • Directed evolution holds potential for further enhancing phage vector capabilities.