Alfa-inhibin and transferrin in human fetal testis

Aim of this research was to investigate the immunohistochemical localization of alfa-inhibin and transferrin in the human fetal testis. In addition, an attempt was made to identify the Sertoli cells in primary cell cultures of fetal testes by the demonstration of the mRNA for transferrin and the presence of the FSH receptor. 15 fetal testes (8-12 weeks) were utilized. The results of this study show: 1. In sections, immunoreactivity for alfa-inhibin was present in the interstitial cells, whereas transferrin was localized in Sertoli cells; 2. After 20 days of primary culture: a) the percentage of immunoreactive cells for both the substances was about 15%; b) the presence of FSH receptor and the expression of transferrin mRNA were observed in 12-15% of cells. These findings suggest: 1. alfa-inhibin and transferrin are present in the human fetal testis; 2. the expression of transferrin mRNA and the presence of the FSH receptor permit the identification of the Sertoli cells in primary cultures of fetal testes (8-12 weeks).