Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Progression after release of obstructive nephropathy.

W Chan1, R J Krieg, K Ward

  • 1Department of Pediatrics, Virginia Commonwealth University, Richmond 23298, USA.

Pediatric Nephrology (Berlin, Germany)
|April 27, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Viral vectors for antimicrobial peptide expression: a new path for crop protection.

Frontiers in microbiology·2026
Same author

Design of a single particle-interferometric reflectance imaging sensor adipo-chip for obesity biomarker screening.

Scientific reports·2025
Same author

Transcriptome profiling of symptomatic vs. asymptomatic grapevine plants reveals candidate genes for plant improvement against trunk diseases.

BMC plant biology·2025
Same author

Autism and Dementia: A Summative Report from the 2nd International Summit on Intellectual Disabilities and Dementia.

Journal of autism and developmental disorders·2025
Same author

Intra and inter-organ communication through extracellular vesicles in obesity: functional role of obesesomes and steatosomes.

Journal of translational medicine·2025
Same author

Cerebral venous thrombosis in children an 18-year review of a Portuguese hospital.

Neurologia·2024

This study establishes a progressive kidney injury model in adult rats after ureteral obstruction reversal. Alpha-tocopherol demonstrated renal protective effects, reducing fibrosis and apoptosis in the injured kidneys.

Area of Science:

  • Nephrology
  • Renal Pathophysiology
  • Pharmacology

Background:

  • Unilateral ureteral obstruction (UUO) in neonatal rats leads to progressive fibrosis.
  • Reversal of UUO (RUUO) in adult rats may offer a model for studying progressive renal injury.
  • Alpha-tocopherol's potential role in modulating fibrogenic pathways warrants investigation.

Purpose of the Study:

  • To establish a progressive renal injury model in adult rats following RUUO.
  • To investigate the effects of alpha-tocopherol on renal fibrosis, apoptosis, and stress markers post-RUUO.
  • To differentiate RUUO-induced injury from compensatory hypertrophy after contralateral nephrectomy (NX).

Main Methods:

  • Adult male Sprague-Dawley rats underwent RUUO, with or without contralateral NX.

Related Experiment Videos

  • Groups received either regular chow or alpha-tocopherol supplementation for 7 or 14 days.
  • Kidney weight/body weight ratio, TGFbeta1 mRNA, TUNEL assay (apoptosis), and HSP-70 staining were assessed.
  • A separate experiment evaluated NX effects with/without alpha-tocopherol.

    • Main Results:

    • RUUO with NX in adult rats induced progressive renal injury, evidenced by increased kidney weight/body weight ratio and apoptosis at 14 days.
    • Alpha-tocopherol supplementation significantly reduced kidney weight/body weight ratio, TGFbeta1 mRNA, and apoptosis at 14 days post-RUUO+NX.
    • Elevated TGFbeta1 mRNA and apoptosis in the RUUO+NX model were significantly attenuated by alpha-tocopherol treatment.
    • NX alone did not induce significant changes in apoptosis or TGFbeta1 mRNA, confirming RUUO as the primary injury driver.

    Conclusions:

    • A progressive renal injury model can be successfully established in adult rats after RUUO and contralateral NX.
    • Alpha-tocopherol exhibits significant renal protective effects in this progressive injury model, mitigating fibrosis and apoptosis.
    • The findings support alpha-tocopherol's therapeutic potential in managing progressive kidney disease.