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Related Experiment Videos

Progression after release of obstructive nephropathy.

W Chan1, R J Krieg, K Ward

  • 1Department of Pediatrics, Virginia Commonwealth University, Richmond 23298, USA.

Pediatric Nephrology (Berlin, Germany)
|April 27, 2001
PubMed
Summary

This study establishes a progressive kidney injury model in adult rats after ureteral obstruction reversal. Alpha-tocopherol demonstrated renal protective effects, reducing fibrosis and apoptosis in the injured kidneys.

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Area of Science:

  • Nephrology
  • Renal Pathophysiology
  • Pharmacology

Background:

  • Unilateral ureteral obstruction (UUO) in neonatal rats leads to progressive fibrosis.
  • Reversal of UUO (RUUO) in adult rats may offer a model for studying progressive renal injury.
  • Alpha-tocopherol's potential role in modulating fibrogenic pathways warrants investigation.

Purpose of the Study:

  • To establish a progressive renal injury model in adult rats following RUUO.
  • To investigate the effects of alpha-tocopherol on renal fibrosis, apoptosis, and stress markers post-RUUO.
  • To differentiate RUUO-induced injury from compensatory hypertrophy after contralateral nephrectomy (NX).

Main Methods:

  • Adult male Sprague-Dawley rats underwent RUUO, with or without contralateral NX.

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  • Groups received either regular chow or alpha-tocopherol supplementation for 7 or 14 days.
  • Kidney weight/body weight ratio, TGFbeta1 mRNA, TUNEL assay (apoptosis), and HSP-70 staining were assessed.
  • A separate experiment evaluated NX effects with/without alpha-tocopherol.

    • Main Results:

    • RUUO with NX in adult rats induced progressive renal injury, evidenced by increased kidney weight/body weight ratio and apoptosis at 14 days.
    • Alpha-tocopherol supplementation significantly reduced kidney weight/body weight ratio, TGFbeta1 mRNA, and apoptosis at 14 days post-RUUO+NX.
    • Elevated TGFbeta1 mRNA and apoptosis in the RUUO+NX model were significantly attenuated by alpha-tocopherol treatment.
    • NX alone did not induce significant changes in apoptosis or TGFbeta1 mRNA, confirming RUUO as the primary injury driver.

    Conclusions:

    • A progressive renal injury model can be successfully established in adult rats after RUUO and contralateral NX.
    • Alpha-tocopherol exhibits significant renal protective effects in this progressive injury model, mitigating fibrosis and apoptosis.
    • The findings support alpha-tocopherol's therapeutic potential in managing progressive kidney disease.