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Structure of the extracellular polysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus 291
E J Faber1, J P Kamerling, J F Vliegenthart
1Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, The Netherlands.
Carbohydrate Research
|April 27, 2001
Abstract:
The lactic acid bacterium Lactobacillus delbrueckii subsp. bulgaricus 291, when grown in skimmed milk, produced 80 mg/L exopolysaccharide with an average molecular mass of 1.4 x 10(3) kDa. Monosaccharide analysis, methylation analysis, MS, and 1D/2D NMR (1H and 13C) studies performed on the native polysaccharide, and on oligosaccharides obtained from a mild acid hydrolysate of the native polysaccharide, showed the polysaccharide to consist of branched pentasaccharide repeating units with the following structure: [structure: see text].