Changes in enzyme and metabolite content of effluent perfusate during preservation of dog kidneys by Collins' method

Enzyme and metabolite content of effuent perfusate has been investigated. One kidney was removed from dogs and preserved by Collin's method. The animals were then bled to a mean arterial blood pressure of 5.26 KPa (40 mm Hg) and maintained at this pressure for 60 min after which the other kidney was removed and preserved in a similar way. 10-ml samples of effluent perfusate were taken immediately, and after 30 min, 1, 2, 3, 4, 6, 8, 24, 48 and 72 h. Acid phosphatase, arylsulphatase "A", ss-glucuronidase, LDH, leucin-aminopeptidase, lactic acid and pyruvic acid were determined by fluorometric methods. It was found that the activity of the enzymes rose parallel with the duration of preservation, the rise was especially great at 48 and 72 h. Activity of LDH and arylsulphatase "A" was significantly higher even at the beginning of preservation in the group of kidneys exposed in vivo to hypotension. This significant difference was present at 24, 48 und 72 h in the activity of all enzymes.