A histological procedure to determine dental age
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Updated: Oct 8, 2026

Systematic Assessment of Mammalian Skull Specimens for Dental and Temporomandibular Joint Pathology
Published on: August 22, 2022
M L Amariti1, M Restori, F De Ferrari
1Institute of Legal Medicine, University of Brescia-Piazzale Spedali Civili 1, 25123, Brescia, Italy.
Abstract:
Sclerosis of dentine has become one of the well established indicators of ageing and age determination. In this study a new technique was reported where a photomicrographic image of a cross section of sclerotic dentine was converted to a grey scale of 256 tones and then reduced to black and white and read by computer using specially developed software. A regression analysis was applied to a sample of 62 teeth (age range 17-84 years) and an age determination within an error limit of 11 years was obtained. Using a Neural Network software however the error was reduced to 8 years.
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