[Determination of avermictin in avermictin tablet by high performance liquid chromatography]

A method for the separation and determination of avermictin in avermictin tablet by reversed-phase high performance liquid chromatography (RP-HPLC) was developed. Operating conditions were as follows: TSK-GEL C18 column, 250 mm x 4.6 mm i.d., MeOH-H2O (75:25, V/V) mobile phase with a flow rate of 1.0 mL/min, and UV detection at 245 nm. The results showed that the active constituents avermictin B1a and B1b can by separated effectively. The relative standard deviations were 2.56% and 1.31%, respectively (n = 6). The method is accurate, rapid and simple, and provides a scientific basis for industrial production and quality control of avermictin tablet.