[Changes of K+ channel current in cerebellar cortical neuron of apoptosis induced with H2O2]

The neurons dissociated from the cerebellar cortex of neonatal SD rat were cultured, while the growth of non-neuron cells were suppressed by Ara-c. Apoptosis of the neurons were induced with H2O2. The changes of Ik were studied during apoptosis using the patch clamp technique in cell-attached configurations. The results are as follows: (1) the Ik amplitudes of the neurons were lower than that of normal at various corresponding clamp voltages; (2) the conductances, mean open time, open probability, long and short opening time constants were all lower, as compared with normal neurons. All these results indicate that the activities of K+ channels on cerebellar neurons decrease during apoptosis.