[Dye coupling between visual cortical (area 17) neurones of adult rat--a study on brain slices]

With intracellular injection of biocytin, forty-nine neurones were successfully labeled in in vitro visual cortex (area 17) slices of the adult rat. Out of the sample dye coupling was found in 21 cases, for each injection the number of the coupled cells varying from 2 to 5 (mean 2.8 +/- 1.1). Eighteen couplings were pyramidal-pyramidal, two were pyramidal-nonpyramidal, and one was seen among non-pyramidal cells. In most cases, the coupled cells were apart from each other with a certain distance among their cell bodies (mean 43.1 +/- 24 microns). The longest distance was up to 635 microns. Most couplings (19/21) were observed within the same lamina; only two in different laminae. The dye couplings were found in laminae II/III, IV and V, but mostly in laminae II/III. We have measured membrane potential and response properties to white matter and intracellular stimulation for the coupled and non-coupled cells; no significant difference was found between them.