[Neural differentiation of Wnt-1 overexpression P19 cells]

Wnt-1 gene plays important roles during the development of the mouse central neural system. and shown a transient expression during neuronal differentiation of P19 EC cells. We transfected Wnt-1 gene into P19 cells and found the Wnt-1 transfected cells could differentiate into neurons without the induction of RA. However, MASH-1, a crucial gene for the early neural differentiation, was expressed later than P19 cells induced by RA.