Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sphingosine 1-phosphate: synthesis and release.

Y Yatomi1, Y Ozaki, T Ohmori

  • 1Department of Laboratory Medicine, Yamanashi Medical University, Japan. yatomiy@res.yamanashi-med.ac.jp

Prostaglandins & Other Lipid Mediators
|April 28, 2001
PubMed
Summary

Platelets store and release abundant sphingosine 1-phosphate (Sph-1-P), a bioactive lipid crucial for cell signaling. This suggests platelets are a key source of extracellular Sph-1-P, impacting vascular biology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk factors for decreased teicoplanin trough concentrations during initial dosing in critically ill patients.

Die Pharmazie·2019
Same author

A simplified chart for determining the initial loading dose of teicoplanin in critically ill patients.

Die Pharmazie·2018
Same author

The impact of external fixation on mortality in patients with an unstable pelvic ring fracture: a propensity-matched cohort study.

The bone & joint journal·2018
Same author

A genome-wide association study identifies two novel susceptibility loci and trans population polygenicity associated with bipolar disorder.

Molecular psychiatry·2017
Same author

Scoring system to predict hemorrhage in pelvic ring fracture.

Orthopaedics & traumatology, surgery & research : OTSR·2016
Same author

Safe zone for transacetabular screw fixation using a Kerboull cross-plate: A CT-scan templating prospective study.

Orthopaedics & traumatology, surgery & research : OTSR·2016

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Lipid Signaling

Background:

  • Sphingosine 1-phosphate (Sph-1-P) functions as both an intracellular second messenger and an extracellular mediator in mammalian cells.
  • Intracellular Sph-1-P signaling relies on stimulation-dependent synthesis, often involving sphingosine (Sph) kinase.
  • Extracellular Sph-1-P's roles necessitate understanding its sources and cell surface receptors.

Purpose of the Study:

  • To investigate the role of blood platelets as a source of extracellular Sph-1-P.
  • To explore the mechanisms behind abundant Sph-1-P storage and release by platelets.

Main Methods:

  • Analysis of Sph-1-P levels in platelets.
  • Investigation of sphingosine kinase activity and Sph-1-P lyase presence in platelets.

Related Experiment Videos

  • Observation of Sph-1-P release from platelets upon stimulation.

    • Main Results:

    • Blood platelets store Sph-1-P in high concentrations.
    • Platelets possess highly active Sph kinase and lack Sph-1-P lyase, contributing to Sph-1-P accumulation.
    • Platelets release significant amounts of Sph-1-P extracellularly upon stimulation.

    Conclusions:

    • Platelets are a major source of extracellular Sph-1-P, particularly in plasma and serum.
    • Platelet-derived Sph-1-P likely plays a significant role in vascular biology.
    • Further research into Sph-1-P regulation and receptor identification is warranted.