Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method to microinject solid neural tracers into deep structures of the brain.

G Marín1, P Henny, J C Letelier

  • 1Departamento de Biología, Universidad de Chile, Casilla 653, Santiago, Chile.

Journal of Neuroscience Methods
|April 28, 2001
PubMed
Summary

Researchers created a new instrument for precise solid neural tracer microinjections into deep brain structures. This tool enables accurate targeting and clear visualization of neural pathways, improving brain research methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Higher rate of pathologic complete response in patients with early-onset locally advanced rectal cancer.

ESMO gastrointestinal oncology·2026
Same author

Functional correlation between cerebellum and basal ganglia: A parkinsonism model.

Neurologia·2024
Same author

Seven Day Safety Net Service.

Acute medicine·2022
Same author

Video self-confrontation as a therapeutic tool in schizophrenia: A randomized parallel-arm single-blind trial.

Schizophrenia research·2022
Same author

Teledentistry and forensic odontology: Cross-sectional observational comparative pilot study.

Forensic science international·2021
Same author

Increased risks of SARS-CoV-2 nosocomial acquisition in high-risk COVID-19 units justify personal protective equipment: a cross-sectional study.

The Journal of hospital infection·2020

Area of Science:

  • Neuroscience
  • Biomedical Engineering

Background:

  • Accurate delivery of neural tracers to deep brain structures is crucial for understanding neural circuitry.
  • Conventional methods using liquid tracers can lead to diffusion and imprecise targeting.

Purpose of the Study:

  • To develop and validate a novel instrument for microinjecting solid neural tracers into deep brain regions.
  • To assess the precision, efficacy, and transport characteristics of solid tracer injections.

Main Methods:

  • Development of a microinjection instrument with a movable internal rod and pressure chamber for solid tracer delivery.
  • Integration of a microelectrode for electrophysiological guidance of injection placement.
  • Testing the instrument by microinjecting DiI and rhodamine crystals into pigeon visual system structures.

Related Experiment Videos

Main Results:

  • Achieved small, well-defined injection sites accurately located in target brain regions.
  • Demonstrated well-developed anterogade and retrograde transport originating selectively from injection sites.
  • Confirmed the instrument's capability for precise delivery of solid neural tracers.

Conclusions:

  • The developed instrument enables reliable microinjection of solid neural tracers into deep brain structures.
  • This method offers an advantageous alternative to conventional liquid tracer injection techniques for certain applications.
  • The instrument enhances the ability to study neural pathways with improved accuracy and selectivity.