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Related Experiment Videos

Mouse mast cell secretory granules can function as intracellular ionic oscillators.

I Quesada1, W C Chin, J Steed

  • 1Instituto de Bioingenieria, Universidad Miguel Hernández, Alicante 03550, Spain.

Biophysical Journal
|April 28, 2001
PubMed
Summary

Calcium (Ca2+) dynamics in mast cell granules show periodic oscillations and release, driven by a conserved intracellular mechanism involving potassium (K+) exchange and inositol 1,4,5-trisphosphate (InsP3) receptors.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Mast cells are crucial in allergic responses, involving regulated calcium signaling.
  • Intracellular calcium stores, like granules, play a vital role in cellular functions.
  • Understanding calcium dynamics is key to deciphering cellular communication and responses.

Purpose of the Study:

  • To directly investigate the dynamics of Ca2+ within isolated mast cell granules.
  • To elucidate the mechanisms underlying Ca2+ oscillations and release in these granules.
  • To compare Ca2+ dynamics in normal and beige mouse mast cell granules.

Main Methods:

  • Utilized fluorescent Ca2+ probes for real-time monitoring.
  • Employed digital photo-sectioning techniques for high-resolution imaging.

Related Experiment Videos

  • Performed experiments on isolated mast cell granules from normal and beige mice.

    • Main Results:

    • Resting intraluminal Ca2+ concentration was measured at approximately 25 microM.
    • Inositol 1,4,5-trisphosphate (InsP3) induced periodic Ca2+ oscillations (approx. 10 microM amplitude, 8-10s period).
    • Ca2+ release from granules was coupled to these luminal oscillations, mediated by a Ca2+/K+ exchange process.

    Conclusions:

    • Mast cell granule Ca2+ dynamics are regulated by a Ca2+/K+ exchange mechanism.
    • Apamin-sensitive Ca2+-sensitive K+ channels (ASK(Ca)) and InsP3-receptors (InsP3-R) are involved in Ca2+ flux.
    • A conserved mechanism for Ca2+ oscillations and release may exist across various intracellular storage compartments.