Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatitis C virus genotyping based on 5' noncoding sequence analysis (Trugene).

P Halfon1, P Trimoulet, M Bourliere

  • 1Département de Virologie, Laboratoire Alphabio, 23 Rue de Friedland, Hôpital Ambroise Paré, 13006, Marseille, France. philippe.halfon@alphabio.fr

Journal of Clinical Microbiology
|April 28, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19 and chronic liver disease: results from the 1219 patients French registry.

Scientific reports·2025
Same author

[Hepatitis E as a zoonosis].

Virologie (Montrouge, France)·2021
Same author

RNA editing blood biomarkers for predicting mood alterations in HCV patients.

Journal of neurovirology·2019
Same author

Efficacy, safety and patient-reported outcomes of ledipasvir/sofosbuvir in NS3/4A protease inhibitor-experienced individuals with hepatitis C virus genotype 1 and HIV coinfection with and without cirrhosis (ANRS HC31 SOFTRIH study).

HIV medicine·2017
Same author

The negative impact of HBV/HCV coinfection on cirrhosis and its consequences.

Alimentary pharmacology & therapeutics·2017
Same author

Open-label study of faldaprevir plus peginterferon and ribavirin in hepatitis C virus genotype 1-infected patients who failed placebo plus peginterferon and ribavirin.

Journal of viral hepatitis·2015
Same journal

Ebola laboratory preparedness at frontline hospitals: can we or can't we?

Journal of clinical microbiology·2026
Same journal

Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

Journal of clinical microbiology·2026
Same journal

Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

Journal of clinical microbiology·2026
Same journal

Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

Journal of clinical microbiology·2026
Same journal

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same journal

Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

Journal of clinical microbiology·2026
See all related articles

Accurate Hepatitis C virus (HCV) genotyping is crucial. This study evaluated Trugene and InnoLiPA assays against NS5B sequencing, finding high concordance for HCV types but variable subtype discrimination.

Area of Science:

  • Hepatology
  • Virology
  • Molecular Diagnostics

Background:

  • Accurate Hepatitis C virus (HCV) genotyping is essential for patient management and epidemiological studies.
  • Existing genotyping methods require validation against reliable molecular techniques.

Purpose of the Study:

  • To evaluate the performance of the Trugene 5'NC and InnoLiPA assays for Hepatitis C virus (HCV) genotyping.
  • To compare these commercial assays against in-house NS5B sequencing for HCV genotyping accuracy.

Main Methods:

  • HCV genotyping was performed on 184 chronic HCV patient samples using Trugene 5'NC and InnoLiPA assays.
  • Samples were also analyzed by sequencing of the NS5B region of the HCV genome after in-house PCR amplification for validation.
  • Genotype distribution was determined, including subtypes like 1a, 1b, 2a, 3a, and 4a.

Related Experiment Videos

Main Results:

  • The Trugene and InnoLiPA assays demonstrated 100% concordance for identifying Hepatitis C virus (HCV) types.
  • Subtype discrimination accuracy was 76% for Trugene and 74% for InnoLiPA.
  • NS5B sequencing revealed a diverse distribution of HCV genotypes and subtypes in the patient cohort.

Conclusions:

  • Both Trugene and InnoLiPA assays are reliable for Hepatitis C virus (HCV) type identification.
  • While accurate for types, their performance in discriminating subtypes varies and may require complementary methods.
  • NS5B sequencing serves as a valuable validation tool for HCV genotyping assays.