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Related Experiment Videos

Comparison of two methods for serotyping Campylobacter spp.

D McKay1, J Fletcher, P Cooper

  • 1Department of Medical Microbiology, Grampian University Hospitals Trust, Foresterhill, Aberdeen AB25 2ZN, Scotland.

Journal of Clinical Microbiology
|April 28, 2001
PubMed
Summary

Penner serotyping is a more effective method for classifying Campylobacter sp. isolates compared to the Laboratory of Enteric Pathogens (LEP) scheme, which left many samples untypeable.

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Area of Science:

  • Microbiology
  • Bacteriology
  • Food Safety

Background:

  • Campylobacter species are significant foodborne pathogens.
  • Accurate serotyping is crucial for epidemiological surveillance and outbreak investigations.
  • Existing serotyping schemes require evaluation for optimal performance.

Purpose of the Study:

  • To compare the efficacy of two serotyping schemes, Penner and Laboratory of Enteric Pathogens (LEP), for Campylobacter sp. isolates.
  • To assess the performance characteristics of each scheme, including discrimination capability and untypeable rates.

Main Methods:

  • Analysis of 3,788 Campylobacter sp. isolates using both Penner and LEP serotyping schemes.
  • Evaluation of antigen-based typing methods for soluble heat-stable antigens.

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Main Results:

  • The LEP serotyping scheme resulted in a significant percentage (36.6%) of untypeable isolates.
  • Greater cross-reactivity was observed with the LEP scheme compared to the Penner scheme.
  • Both schemes demonstrated similar relative discrimination capabilities.

Conclusions:

  • The Penner serotyping scheme is superior due to its lower rate of untypeable isolates and reduced cross-reactivity.
  • Penner serotyping better meets the criteria for a reliable bacterial typing method for Campylobacter.
  • The findings support the use of Penner serotyping for improved Campylobacter surveillance.