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Evaluation of a reformulated CHROMagar Candida
M A Jabra-Rizk1, T M Brenner, M Romagnoli
1Department of Oral Medicine, Dental School, University of Maryland, 666 W. Baltimore St., Baltimore, MD 21201, USA. mrizk@umaryland.edu
Journal of Clinical Microbiology
|April 28, 2001
Summary
The new CHROMagar Candida medium effectively identifies yeasts, showing comparable recovery to the original formulation. It improves differentiation between Candida albicans and Candida dubliniensis by producing lighter green colonies for C. albicans.
Area of Science:
- Medical Microbiology
- Diagnostic Mycology
- Clinical Laboratory Science
Background:
- CHROMagar Candida is a key differential medium for yeast identification.
- Becton Dickinson recently reformulated CHROMagar Candida.
- Evaluating new diagnostic media is crucial for clinical laboratories.
Purpose of the Study:
- To compare the performance of the new CHROMagar Candida formulation against the original.
- To assess recovery, growth, and colony color for yeast identification.
- To evaluate the utility in differentiating key Candida species.
Main Methods:
- Tested stock cultures and clinical specimens with both original and new CHROMagar Candida media.
- Included 90 yeast stock isolates (9 species) and 522 clinical specimens.
- Assessed yeast recovery rates, growth characteristics, and colony morphology/color.
Main Results:
- Both media demonstrated equivalent yeast recovery rates and growth.
- Candida albicans produced lighter green colonies on the new medium.
- Candida dubliniensis showed consistent dark green colonies on both media, aiding differentiation.
Conclusions:
- The new CHROMagar Candida formulation is comparable to the original for yeast recovery and identification.
- The altered colony color of Candida albicans on the new medium enhances differentiation from Candida dubliniensis.
- The reformulated medium is suitable for presumptive yeast identification and detection in clinical samples.