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Updated: Jun 29, 2026
Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
Published on: January 14, 2014
[Morphometric investigation on hypoxic structural remodeling of intraacinar pulmonary arteries]
1Department of Pathology, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037.
Objective:
To observe the inhibitory effects of Radix Astragali (RA) on hypoxic structural remodeling of intraacinar pulmonary arteries (IAPA) and pulmonary hypertension (PHT).
Method:
60 rats were divided into H (hypoxia) group (n = 20 rats) H + RA group (n = 20) and normal control (C) group (n = 20). The rats of H group and H + RA group were fed in hypoxic environment under normal atmospheric pressure (10% O2, 10 hours/day). On the 15th and 30th day of hypoxia, right ventricular systolic pressure (RVSP) and right ventricle hypertrophy index (RVHI) were measured and pulmonary vessel changes were studied under light and electron microscope with morphometric analysis.
Result:
Compared with H + RA group on 30th day, RVSP in H was 1.8 time as high as that of H + RA(P < 0.05); RVHI in H was 1.3 time as high as that of H + RA (P < 0.05); the media thickness of IAPA in H was 2.3 time as high as that of H + RA (P < 0.05); the density of adventitial fibroblasts of IAPA was 13.1 +/- 0.3 (H) and 7.6 +/- 0.2 (H + RA) (P < 0.05).
Conclusion:
Preserving the IAPA wall cells and dilating IAPA by RA may play an important role in inhibiting remodeling of IAPA and PTH.
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