[Determination of antimicrobial preservative dimethyl fumarate by high performance liquid chromatography]

This paper described a HPLC method for determining dimethyl fumarate (DMF), a new antimicrobial preservative. Chromatographic conditions were as follows: Micropak MGH 5 mu cap column, 4.00 mm x 300.0 mm EtOH-H2O (90:10, V/V) eluent, and UV detector at 216 nm. In the range of 0.036-2.96 micrograms, the sample size and peak area showed a linear relationship with regression equation, Y = 3.215 x 10(-6)X - 0.04797 and r = 0.9996. Standard deviation was 3.2 x 10(-4).