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[Study of protein separation by reversed-phase high performance liquid chromatography]
1State Key Laboratory of Dye and Surfactant Fine Chemicals, Dalian University of Technology, Dalian, 116012.
Se Pu = Chinese Journal of Chromatography
|May 1, 2001
Abstract:
Reversed-phase high performance liquid chromatography (RP-HPLC) of protein on large pore silica (20-30 nm) n-alkyl-bonded(C4 and C5) phase columns was studied using a trifluoroacetic acid (TFA)/2-propanol mobile phase system. The influence of concentration of organic modifier (2-propanol) and ion-pairing acid (TFA) on the protein retention time was examined. The results showed that the plot of the retention time vs-2-propanol concentration in mobile phase was U-shaped. The mechanism of protein in reversed-phase high performance liquid chromatography is also discussed.