Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flagellin polymerisation control by a cytosolic export chaperone.

F Auvray1, J Thomas, G M Fraser

  • 1Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

Journal of Molecular Biology
|May 1, 2001
PubMed
Summary

A cytosolic protein, FliS, specifically facilitates bacterial flagellin (FliC) export by binding to FliC subunits. This chaperone action prevents premature polymerization, ensuring proper flagellar filament assembly.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementation of non-medical prescribing by therapeutic radiographers: Views of prescribers and service managers in devolved UK health systems.

Radiography (London, England : 1995)·2026
Same author

Corrigendum to "Healthcare-worker-associated outbreak of Panton-Valentine-leukocidin-producing meticillin-sensitive Staphylococcus aureus in a large neonatal unit in London: successful targeted suppression therapy following failure of mass suppression therapy" [J Hosp Infect 122 (2022) 148-156].

The Journal of hospital infection·2025
Same author

Reducing inappropriate vitamin D testing: an analysis of three sequential diagnostic stewardship interventions and the carbon footprint implications.

Clinical biochemistry·2025
Same author

GLP-1 receptor agonist use for obesity in service personnel.

BMJ military health·2025
Same author

Digital proficiency and educational needs of Portuguese radiation therapists.

Radiography (London, England : 1995)·2025
Same author

Perception of medical imaging educators on the addition of AI education to the medical imaging curriculum: A cross-sectional survey.

Radiography (London, England : 1995)·2025

Area of Science:

  • Microbiology
  • Molecular Biology
  • Structural Biology

Background:

  • Bacterial flagellar assembly involves the polymerization of approximately 20,000 flagellin (FliC) subunits.
  • The precise mechanism of flagellin export and subunit stabilization remains an area of investigation.

Purpose of the Study:

  • To investigate the role of the cytosolic protein FliS in the export and polymerization of Salmonella flagellin (FliC).
  • To elucidate the interaction between FliS and FliC and its impact on flagellar filament formation.

Main Methods:

  • In vitro assembly of stable FliS-FliC complexes.
  • Analysis of FliS binding to the C-terminal helical domain of FliC.
  • Assessment of FliS's ability to prevent FliC polymerization.

Related Experiment Videos

Main Results:

  • FliS specifically facilitates the export of Salmonella flagellin (FliC).
  • FliS binds to the C-terminal helical domain of FliC, stabilizing subunit interactions.
  • FliS homodimers efficiently bind FliC monomers, forming stable complexes in vitro.
  • FliS acts as a substrate-specific chaperone, preventing premature FliC subunit interactions in the cytosol.
  • FliS inhibits in vitro polymerization of FliC into filaments.

Conclusions:

  • FliS is a crucial chaperone for bacterial flagellin export and polymerization.
  • FliS binding to FliC prevents premature subunit interactions, ensuring ordered flagellar filament assembly.