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Published on: January 12, 2015
Use of Green Fluorescent Protein in mouse embryos
1Wellcome/CRC Institute, Tennis Court Road, Cambridge CB2 1QR, United Kingdom. mzg@mole.bio.cam.ac.uk
Methods (San Diego, Calif.)
|May 1, 2001
Summary
Green Fluorescent Protein (GFP) is a versatile cell marker. Modified GFP enhances solubility for gene expression, cell lineage, and protein localization studies in mammalian cells and embryos.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Green Fluorescent Protein (GFP) is a widely used cell marker.
- Mutations improved GFP solubility for mammalian cell applications.
- GFP enables gene expression, cell lineage, and protein localization studies.
Purpose of the Study:
- To outline the use of Green Fluorescent Protein (GFP) mRNA and cDNA.
- To demonstrate GFP applications in mouse cell lineage studies.
- To characterize protein behavior within embryos using GFP.
Main Methods:
- Utilizing Green Fluorescent Protein (GFP) mRNA and cDNA.
- Employing mutations to enhance GFP solubility for mammalian cells.
- Visualizing fluorescent protein in living cells without photodamage.
Main Results:
- Green Fluorescent Protein (GFP) demonstrated utility in mammalian gene expression studies.
- GFP facilitated cell lineage tracing in mouse models.
- Protein localization and behavior within embryos were characterized using GFP.
Conclusions:
- Green Fluorescent Protein (GFP) is a powerful tool for biological research.
- Optimized GFP variants are effective for diverse applications in mammalian systems.
- GFP enables detailed studies of cellular processes and embryonic development.
