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Updated: Oct 7, 2026

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
Published on: August 15, 2025
Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media
1Fish Health Unit, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Launceston, Australia. Teresa.Wilson@dpiwe.tas.gov.au
Aims:
To create a fast, sensitive and inexpensive high-throughput method for the extraction of bacterial genomic DNA from selective-enrichment culture media.
Methods And Results:
Lysis of bacteria was achieved using guanidinium isothiocyanate, and DNA was extracted using 96-well glass microfibre filtration plates. Extraction-PCR detected the presence of 1 cfu Yersinia ruckeri and 16 cfu Lactococcus garvieae 200 microl(-1) sample of selective-enrichment medium.
Conclusion:
An efficient method for high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media was achieved.
Significance And Impact Of The Study:
This method enables detection of covert bacterial infections in fish. The simultaneous extraction of large numbers of samples allows for its use in bacterial monitoring programmes and quarantine.

