Vasoactive intestinal polypeptide in neuroglia? Immunoelectron microscopic localization in astrocytes of the rat...

The dorsal region of the rat interpeduncular nucleus (IPN) was found highly immunoreactive for vasoactive intestinal polypeptide (VIP). This area appeared as a cap-like structure at the midcaudal level of the nucleus. Unlike other brain areas, however, VIP immunoreactivity within the "cap" appeared vaguely punctuate with no light microscopically identifiable cell structures. Ultrastructurally, a dense meshwork of VIP-immunopositive bouton-laden axons was revealed. Labeled neuronal perikarya were not encountered. Some lightly immunoreactive dendrites were observed. In addition, immunopositive glial profiles were frequently seen. Perikarya and numerous fine processes, occasionally perivascular, identified as astroglia by established ultrastructural criteria, exhibited VIP immunoreactivity. Constant feature of the peptide immunolocalization was the predilection for the intermediate filament bundles of astrocytic perikarya and processes. This was usually accompanied by a thick coating of the inner aspect of glial plasmalemma and, in perikarya, by highly reactive vesicular profiles. Glial immunopositive elements were never seen beyond the boundaries of the diffuse "cap." In view of the repertoire of metabolic, neurotrophic, and neuroprotective mechanisms in which VIP neurons are involved in conjunction with astroglia, the presence of VIP-immunoreactive astrocytes in a circumscribed area, confirms the heterogeneity of astrocyte populations.