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Cloned mice derived from somatic cell nuclei
1Department of Applied Biological Science, Nihon University College of Bioresource Sciences.
Human Cell
|May 2, 2001
Summary
Somatic cell nuclear transfer in animals was investigated using microinjection and electrofusion. Microinjection produced cloned embryos that developed to the 8-cell stage, with some resulting in fetuses, though fragmentation rates were higher than with electrofusion.
Area of Science:
- Animal cloning
- Reproductive biology
- Developmental biology
Background:
- Somatic cell nuclear transfer (SCNT) has enabled the cloning of various animals, including sheep and mice.
- Cumulus cells, fibroblasts, and Sertoli cells are established nuclear donors for SCNT.
- Comparing nuclear transfer techniques is crucial for improving cloning efficiency.
Purpose of the Study:
- To compare the efficiency of microinjection and electrofusion methods for SCNT in producing cloned embryos.
- To evaluate the developmental potential of SCNT embryos generated by each method.
Main Methods:
- SCNT was performed using either microinjection or electrofusion of donor cell nuclei into enucleated oocytes.
- Oocyte enucleation was achieved using a micromanipulator.
- Reconstituted embryos were activated using St2+ and assessed for development and fragmentation.
Main Results:
- Cloned embryos produced by microinjection developed to the 8-cell stage.
- Microinjection resulted in a higher rate of embryo fragmentation compared to electrofusion.
- Transfer of microinjected embryos led to the observation of 9 fetuses at 14 days post coitum.
Conclusions:
- Both microinjection and electrofusion are viable methods for SCNT.
- Electrofusion may offer advantages in reducing embryo fragmentation.
- Further optimization of microinjection techniques could enhance developmental success in animal cloning.