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Cloned mice derived from somatic cell nuclei.

K Hosaka1, S Ohi, A Ando

  • 1Department of Applied Biological Science, Nihon University College of Bioresource Sciences.

Human Cell
|May 2, 2001
PubMed
Summary
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Somatic cell nuclear transfer in animals was investigated using microinjection and electrofusion. Microinjection produced cloned embryos that developed to the 8-cell stage, with some resulting in fetuses, though fragmentation rates were higher than with electrofusion.

Area of Science:

  • Animal cloning
  • Reproductive biology
  • Developmental biology

Background:

  • Somatic cell nuclear transfer (SCNT) has enabled the cloning of various animals, including sheep and mice.
  • Cumulus cells, fibroblasts, and Sertoli cells are established nuclear donors for SCNT.
  • Comparing nuclear transfer techniques is crucial for improving cloning efficiency.

Purpose of the Study:

  • To compare the efficiency of microinjection and electrofusion methods for SCNT in producing cloned embryos.
  • To evaluate the developmental potential of SCNT embryos generated by each method.

Main Methods:

  • SCNT was performed using either microinjection or electrofusion of donor cell nuclei into enucleated oocytes.
  • Oocyte enucleation was achieved using a micromanipulator.

Related Experiment Videos

  • Reconstituted embryos were activated using St2+ and assessed for development and fragmentation.

    • Main Results:

    • Cloned embryos produced by microinjection developed to the 8-cell stage.
    • Microinjection resulted in a higher rate of embryo fragmentation compared to electrofusion.
    • Transfer of microinjected embryos led to the observation of 9 fetuses at 14 days post coitum.

    Conclusions:

    • Both microinjection and electrofusion are viable methods for SCNT.
    • Electrofusion may offer advantages in reducing embryo fragmentation.
    • Further optimization of microinjection techniques could enhance developmental success in animal cloning.