Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peroxisomal proteins in rat gametes.

C Figueroa1, M E Kawada, L P Véliz

  • 1Department of Cell and Molecular Biology, Faculties of Biological Sciences and Medicine, P. Catholic University of Chile, Santiago, Chile.

Cell Biochemistry and Biophysics
|May 2, 2001
PubMed
Summary

Mammalian oocytes contain peroxisome-like structures, supporting the organelle division hypothesis for peroxisome biogenesis. Rat sperm lack these structures, with catalase found in the cytosol.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HLA-DRB1*15:01 drives sex- and age-dependent microglial activation and neuroimmune signaling.

bioRxiv : the preprint server for biology·2026
Same author

<i>Anisakis</i> spp. larvae in black scabbardfish (<i>Aphanopus carbo</i>) muscle: Consumer health risk and recommendations along the value chain.

Food and waterborne parasitology·2025
Same author

Preliminary observations on muscle distribution of <i>Anisakis</i> sp. L3 in European hake off Southwest Ireland.

Food and waterborne parasitology·2025
Same author

Signatures of Endosymbiosis in Mitochondrial Genomes of Rhabdocoel Flatworms.

Molecular ecology·2025
Same author

TRALI: A differential diagnosis to keep in mind.

Pulmonology·2023
Same author

Tuberculosis in Spain: An opinion paper.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2023

Area of Science:

  • Cell Biology
  • Organelle Biogenesis
  • Reproductive Biology

Background:

  • Peroxisomes are vital subcellular organelles.
  • Peroxisome biogenesis is thought to occur via division of pre-existing peroxisomes.
  • This implies peroxisomes must be present in mammalian gametes.

Purpose of the Study:

  • To investigate the presence and location of peroxisomal proteins in rat sperm and oocytes.
  • To test the hypothesis of peroxisome biogenesis through organelle division in mammalian reproduction.

Main Methods:

  • Fractionation experiments were performed on rat gametes.
  • Indirect immunofluorescence was used to detect peroxisomal proteins.
  • Antisera against total peroxisomal proteins were utilized.

Related Experiment Videos

Main Results:

  • Peroxisome-like structures were identified in rat oocytes using immunofluorescence.
  • These structures were not detected in rat sperm.
  • Catalase was found in the cytosol of rat sperm.

Conclusions:

  • Provides the first evidence of peroxisome-like structures in mammalian oocytes.
  • Supports the hypothesis that peroxisomes arise from the division of pre-existing organelles.
  • Suggests differential distribution of peroxisomes in mammalian gametes.