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Updated: Oct 7, 2026

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
Published on: September 9, 2021
Peroxisomal beta-oxidation enzymes
1Department of Biochemistry, Shinshu University School of Medicine, Matsumoto, Nagano 390, Japan.
Abstract:
Peroxisomal fatty acid oxidation enzymes are summarized in comparison to their mitochondrial counterparts. The peroxisomal enzymes involved in the beta-oxidation spiral are schematically classified into two groups. The first group consists of hitherto purified and characterized classical enzymes: palmitoyl-CoA oxidase, the L-bifunctional protein, and 3-ketoacyl-CoA. These enzymes are inducible and act on the straight chain substrates. The second group consists of recently identified enzymes, branched-chain oxidase, the D-bifunctional protein, and sterol-carrier protein x, which catalyze four reactions of beta-oxidation cycle. These are noninducible and act on branched-chain substrates.
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