Related Experiment Video
Updated: Oct 7, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles
Published on: March 2, 2020
Protein-binding polynomials
1Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA. poland@jhunix.hcf.jhu.edu
Abstract:
Using a new method recently published for analyzing the binding isotherms of biopolymers (Poland, 2000a), we calculate the complete binding polynomials for lysozyme, insulin, and serum albumin from published titration data. These three proteins have, respectively, 22, 32, and 184 dissociable protons and hence are represented by series in powers of the hydrogen ion concentration with the highest powers in the series being the numbers just indicated. Given the complete binding polynomial, the distribution function giving the concentration of all states of proton binding can then be calculated at any pH.
Related Concept Videos
Introduction to Polynomial Functions
The Equilibrium Binding Constant and Binding Strength
The Equilibrium Binding Constant and Binding Strength
Physiological Pharmacokinetic Models: Assumption with Protein Binding
Drug Distribution: Plasma Protein Binding
Fundamental Theorem of Algebra
