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Disk diffusion method for fluconazole susceptibility testing of Candida albicans strains
M Yücesoy1, N S Guldaş, N Yuluğ
1Dokuz Eylül University, School of Medicine, Department of Microbiology and Clinical Microbiology, Izmir, Turkey. mineyucesoy@hotmail.com
Journal of Chemotherapy (Florence, Italy)
|May 2, 2001
Summary
The disk diffusion method is a useful screening tool for fluconazole susceptibility in Candida albicans. RPMI 1640, yeast nitrogen base, and Mueller Hinton methylene blue agars showed the best agreement with microdilution methods.
Area of Science:
- Medical Microbiology
- Antifungal Susceptibility Testing
Background:
- Candida albicans is a common cause of fungal infections.
- Accurate susceptibility testing is crucial for effective antifungal treatment.
- Fluconazole is a widely used antifungal agent.
Purpose of the Study:
- To evaluate the disk diffusion method for fluconazole susceptibility testing against Candida albicans.
- To compare different agar media for their effectiveness in disk diffusion testing.
- To determine the reliability of disk diffusion as a screening technique.
Main Methods:
- Evaluated 108 clinical isolates of Candida albicans.
- Used both microdilution and disk diffusion methods.
- Tested disk diffusion on RPMI 1640 agar, yeast nitrogen base agar, Sabouraud dextrose agar, Mueller Hinton agar, and Mueller Hinton methylene blue agar.
- Incubated disk diffusion tests for 24 and 48 hours.
Main Results:
- RPMI 1640 agar and yeast nitrogen base agar showed the best correlation with microdilution at 24 hours.
- Mueller Hinton methylene blue agar demonstrated strong agreement at both 24 and 48 hours.
- High agreement (87-96%) between disk diffusion and microdilution was observed with specific media.
Conclusions:
- Disk diffusion using RPMI 1640, yeast nitrogen base, or Mueller Hinton methylene blue agar is a useful, rapid, and reliable screening method.
- This method aids in assessing fluconazole susceptibility in Candida albicans strains.
- Optimized media selection enhances the accuracy of disk diffusion testing.