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Updated: Aug 3, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Identification of an intragenic integration site for foreign gene expression in recombinant Streptococcus gordonii
T C Bolken1, C A Franke, G O Zeller
1SIGA Research Laboratories, Corvallis, Oregon 97333, USA.
Abstract:
A new intragenic chromosomal integration site within the lacG gene of the lac operon has been identified in Streptococcus gordonii for use in the expression of foreign genes. Introduction of a portion of the Streptococcus pyogenes emm6 gene into the lacG locus resulted in the lactose-inducible surface expression of the S. pyogenes M6 protein. This result demonstrates the ability to modulate the in vitro or in vivo expression of a foreign gene in a S. gordonii recombinant using a biosynthetic metabolite.
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