Quantitative thin-layer spectrodensitometric determination of the components of polyene macrolide antibiotice...

A direct spectrodensitometric method for quantitating the components of polyene macrolide complexes after separation by thin-layer chromatography is described. Resolution of the components of the candidin and candihexin complexes was good up to 2.5 and 10 mu-g/spot, respectively. The peak areas were linear with the amount spotted up to the same levels. Maximum peak areas for the components of the candidin and candihexin complexes were obtained using light wavelengths of 360 and 340 nm, respectively. Spotting errors rather than instrumental parameters were responsible for the variance of repeated determinations. Minimal relative standard deviation values were found at intermediate concentration levels in the linear range.