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Related Concept Videos

  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Increased Myocardial Grp94 Amounts During Sustained Atrial Fibrillation: A Protective Response?
  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Increased Myocardial Grp94 Amounts During Sustained Atrial Fibrillation: A Protective Response?

    • Related Experiment Videos

    Increased myocardial GRP94 amounts during sustained atrial fibrillation: a protective response?

    M Vitadello1, J Ausma, M Borgers

    • 1CNR Unit for Muscle Physiology and Physiopathology, Department of Biomedical Sciences, University of Padova, Padova, Italy.

    Circulation
    |May 23, 2001

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    Chronic atrial fibrillation leads to increased glucose-regulated protein GRP94 in heart cells. This GRP94 increase is reversible, suggesting a protective cellular response in fibrillating atria.

    Related Experiment Videos

    Area of Science:

    • Cardiovascular Biology
    • Cellular Stress Response
    • Molecular Cardiology

    Background:

    • Atrial fibrillation is associated with structural and phenotypic changes in cardiomyocytes.
    • Glucose-regulated protein GRP94 is crucial for cell viability and its role in atrial fibrillation is unclear.

    Purpose of the Study:

    • To investigate changes in GRP94 expression in atrial cardiomyocytes during chronic atrial fibrillation.
    • To determine if GRP94 levels are altered in human and animal models of atrial fibrillation.

    Main Methods:

    • Analysis of GRP94 expression in atrial myocardium from goats and humans using immunologic approaches.
    • Induction and maintenance of atrial fibrillation in goats for up to 16 weeks, followed by cardioversion.
    • Immunohistochemical analysis to confirm the location of GRP94 increase within cardiomyocytes.

    Main Results:

    • GRP94 levels doubled in goat atrial myocytes after 4-16 weeks of fibrillation, returning to normal after cardioversion.
    • Increased GRP94 was also observed in human fibrillating atrial samples.
    • Unlike calreticulin, inducible HSP70 increased in fibrillating atria, suggesting a stress response.

    Conclusions:

    • A significant, reversible increase in GRP94 occurs in atrial myocytes during chronic atrial fibrillation.
    • This GRP94 upregulation may represent a protective cellular mechanism in response to fibrillation.
    • The findings highlight GRP94's potential role in the adaptive response of cardiomyocytes to stress.