Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional interplay between type I collagen and cell surface matrix metalloproteinase activity.

S M Ellerbroek1, Y I Wu, C M Overall

  • 1Department of Cell and Molecular Biology, Northwestern University Medical School, 303 E. Chicago Ave., Chicago, IL 60611, USA.

The Journal of Biological Chemistry
|May 2, 2001
PubMed
Summary

Ovarian cancer cells activate pro-matrix metalloproteinase (pro-MMP)-2 via beta(1) integrin clustering, a process crucial for collagen invasion. This activation is mediated by membrane-type 1 (MT1)-MMP, highlighting its role in cancer cell motility.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MMP8 increases tongue carcinoma cell-cell adhesion and diminishes migration via cleavage of anti-adhesive FXYD5.

Oncogenesis·2021
Same author

High Ratio of Early Postoperative Calcitonin to Preoperative Calcitonin Could be a Novel Indicator of Poor Prognosis in Patients with Biochemical Incomplete Responses in Sporadic Medullary Thyroid Cancer.

Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·2021
Same author

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling.

BMC cell biology·2018
Same author

The Human Odontoblast Cell Layer and Dental Pulp Proteomes and N-Terminomes.

Journal of dental research·2017
Same author

Cadherin composition and multicellular aggregate invasion in organotypic models of epithelial ovarian cancer intraperitoneal metastasis.

Oncogene·2017
Same author

A Novel 1,2-Dihydroquinoline Anticancer Agent and Its Delivery to Tumor Cells Using Cationic Liposomes.

Anticancer research·2016

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • Type I collagen stimulates pro-matrix metalloproteinase (pro-MMP)-2 activation in ovarian cancer cells through beta(1) integrin receptor clustering.
  • The precise cellular and biochemical mechanisms governing MMP processing during this activation remain incompletely understood.

Purpose of the Study:

  • To elucidate the specific cellular and biochemical events involved in collagen-induced pro-MMP-2 activation by ovarian cancer cells.
  • To investigate the role of integrin subtypes and membrane-type 1 (MT1)-MMP in this process and its contribution to cellular invasion.

Main Methods:

  • Utilized DOV13 ovarian cancer cells and type I collagen matrices.
  • Employed antibody-induced integrin clustering (alpha(2)beta(1) and alpha(3)beta(1)) to study pro-MMP-2 activation.

Related Experiment Videos

  • Analyzed cell surface expression and localization of MT1-MMP.
  • Assessed cellular invasion assays in the presence of inhibitors and modified collagen.

    • Main Results:

    • Collagenolysis is not required for pro-MMP-2 activation; intact collagen binding is essential.
    • Alpha(3)beta(1) integrin clustering significantly enhances pro-MMP-2 activation, while alpha(2)beta(1) has minimal effect.
    • Integrin clustering increases MT1-MMP cell surface expression and co-localization with integrin complexes.
    • MT1-MMP undergoes autolysis on the cell surface, independent of active MMP-2.
    • Cellular invasion of collagen matrices depends on collagenase activity and is inhibited by TIMP-2 and collagenase-resistant collagen.

    Conclusions:

    • Integrin-mediated MT1-MMP activity is a critical determinant of ovarian cancer cell invasion through type I collagen.
    • The findings provide a mechanistic insight into how MT1-MMP activity is regulated during collagen degradation and cellular invasion.