Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential splicing of transcripts encoding the orphanin FQ/nociceptin precursor.

J Arjomand1, C J Evans

  • 1Neuroscience Interdepartmental Program, UCLA, Los Angeles, CA 90024-1759, USA.

Journal of Neurochemistry
|May 2, 2001
PubMed
Summary

Researchers found alternative splicing in human and rat preproOFQ/N transcripts, similar to mice. Novel human variants lacking exon 2 were identified, potentially altering protein processing and cellular trafficking of Orphanin FQ (OFQ/N).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "The thymus: what's normal and what's not? Problem-solving with MRI" [Clin Radiol 78 (2024) 885-894].

Clinical radiology·2024
Same author

Physiotherapy for people with dementia: a Call to Action for the development of clinical guidelines.

Physiotherapy·2024
Same author

The thymus: what's normal and what's not? Problem-solving with MRI.

Clinical radiology·2023
Same author

Evidence against altered excitatory/inhibitory balance in the posteromedial cortex of young adult APOE E4 carriers: A resting state <sup>1</sup>H-MRS study.

Neuroimage. Reports·2023
Same author

Influences on emergency department attendance among frail older people with deteriorating health: a multicentre prospective cohort study.

Public health·2021
Same author

Processes of consent in research for adults with impaired mental capacity nearing the end of life: systematic review and transparent expert consultation (MORECare_Capacity statement).

BMC medicine·2020

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • Orphanin FQ/nociceptin (OFQ/N) is a peptide agonist for the NOP receptor, synthesized from a precursor protein, preproOFQ/N.
  • Alternative splicing of the mouse OFQ/N transcript between exons 3 and 4 has been previously reported, leading to precursor proteins with varied C-termini.

Purpose of the Study:

  • To investigate alternative splicing of preproOFQ/N transcripts in humans and rats.
  • To identify novel human splice variants and analyze their potential impact on protein structure and function.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) was used to analyze preproOFQ/N transcripts.
  • In vitro translation of cRNAs was performed to study the products of novel splice variants.

Related Experiment Videos

Main Results:

  • Alternative splicing between exons 3 and 4 of preproOFQ/N transcripts was confirmed in humans and rats.
  • Two novel human preproOFQ/N splice variants were identified, characterized by the excision of exon 2 and alternative splicing between exons 3 and 4.
  • In vitro translation of exon 2-deficient transcripts produced shorter proteins initiated from an alternative methionine within exon 3, lacking a signal peptide.

Conclusions:

  • The identified alternative splicing events in human and rat preproOFQ/N transcripts suggest conserved regulatory mechanisms.
  • Novel human splice variants lacking exon 2 may lead to proteins with altered signal peptide sequences, impacting cellular localization and processing.
  • These findings contribute to understanding the molecular diversity and regulation of OFQ/N precursor protein production.